sf9 questions and creator how is the data collected

melanogaster and divided into two categories: initiator caspases (Dronc, Dredd and dream) and effector caspases (Drice, Dcp-1, decay and Damm) 11. The occurrence of apoptosis process is mainly dependent on cysteine proteases called caspases. Among insect, apoptotic mechanisms of Drosophila melanogaster is more comprehensive. Therefore, the abundant sequence data generated using the NGS technique could be an abundant resource for deep and systematic studies in apoptosis.Īpoptosis is an autonomic-ordered cell death process that independently eliminates superfluous or unwanted cells to maintain the balance of homeostasis in multicellular organisms in response to internal and external stimuli, such as hormones, viruses, UV rays, botanical pesticides, etc. Next-generation sequencing (NGS) technology is a revolutionary change over traditional sequencing, which has been widely used for de novo genome sequencing, re-sequencing, small RNA sequencing and SNP discovery 7. Interestingly, reflecting inerratic characters and easy culture 4, Sf9 cells are also regarded as an ideal model organism for cytotoxicity screening and apoptosis research, and although apoptosis in Sf9 cells has been extensively studied, there is deficient research on its comprehensive genomic resource 5, 6. Sf9 cells, derived from the immature ovaries of fall armyworm moth ( Spodoptera frugiperda) pupae 1, 2, have become one of the most widely used insect cell lines for eukaryotic protein expression with the advantages of high protein expression and easy manipulation 3. In addition, the preliminary results revealed that caspase-dependent or caspase-independent apoptotic pathways could function in azadirachtin-induced apoptosis in Sf9 cells. These data suggested that both intrinsic and extrinsic apoptotic signal pathways comprising the identified potential apoptosis-related genes were potentially active in S. Furthermore, Western blot analysis showed that six putative apoptosis-related proteins were upregulated after being treated with azadirachtin while the protein Bcl-2 were downregulated. Fragments of potential candidate apoptosis-related genes were cloned, and the mRNA expression patterns of ten identified genes regulated by azadirachtin were examined using qRT-PCR.

In the present study, the complete transcriptome data for Sf9 cell line was accomplished using Illumina sequencing technology, and 97 putative apoptosis-related genes were identified through BLAST and KEGG orthologue annotations. However, the lack of genomic data has hindered a deeper investigation of apoptosis in Sf9 cells at a molecular level. The results of a previous study indicated the toxicity and apoptosis induction of azadirachtin in Spodoptera frugiperda Sf9 cells.

As an important botanical pesticide, azadirachtin demonstrates broad insecticidal activity against many agricultural pests.

Sf9 questions and creator how is the data collected